Aequorin-expressing yeast emits light under electric control.
نویسندگان
چکیده
In this study, we show the use of direct external electrical stimulation of a jellyfish luminescent calcium-activated protein, aequorin, expressed in a transgenic yeast strain. Yeast cultures were electrically stimulated through two electrodes coupled to a standard power generator. Even low (1.5 V) electric pulses triggered a rapid light peak and serial light pulses were obtained after electric pulses were applied periodically, suggesting that the system is re-enacted after a short refraction time. These results open up a new scenario, in the very interphase between synthetic biology and cybernetics, in which complex cellular behavior might be subjected to electrical control.
منابع مشابه
Oscillations of calcium ion concentrations in Physarum polycephalum
Aequorin is a photoprotein which emits light in response to changes in free calcium concentration. When aequorin was microinjected into plasmodia of Physarum polycephalum, light emission varied in synchrony with the motile oscillations of the organisms. Therefore, movement is correlated which changes in the concentration of free calcium.
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The luminescent protein aequorin from the jellyfish Aequoria victoria emits light by an intramolecular reaction in the presence of a trace amount of Ca(2+). In order to understand the mechanism of the reaction, a study of structure-function relationships was undertaken with respect to modifying certain of its amino acid residues. This was done by carrying out oligonucleotide-directed site-speci...
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The photoprotein aequorin emits light by an intramolecular reaction when Ca2+ is added under either aerobic or anaerobic conditions. Previously reported evidence has indicated two possibilities: (i) the functional group of aequorin is coelenterazine itself, a compond that plays key roles in the bioluminescence of various other types of organisms, or (ii) it is the enolized form of this compound...
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Aequorin is a photoprotein which emits blue light on binding, with high affinity (m 10 p~), to calcium (Ca2+) [I]. Aequorin is composed of 189-196 amino-acids, with three EF hand structures, characteristic of Ca2+ bindina sites C2.31. Aeauorin has been used as a-CaZ+ indicator in-a variety of biological systems [l]. The aim of this study was to engineer aequorin in order to measure Ca2+ changes...
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عنوان ژورنال:
- Journal of biotechnology
دوره 152 3 شماره
صفحات -
تاریخ انتشار 2011